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cDNA was then used as template for RT-qPCR with a QuantStudio 3 Real-Time PCR system (Applied Biosystems) using PowerUP SYBR Green Master Mix (Applied Biosystems) and 500 nM of target gene specific forward and reverse primers, using the recommended fast cycling mode
Their method of delivery through the nasal passage offers a simple, needle-free solution that many find appealing
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